.Johnson & Johnson is currently seeking a Senior Scientist, Cell Line Development to join our Cell Engineering & Analytical Sciences group within the API-Proteins organization located in Spring House, PA.At Johnson & Johnson, we believe health is everything.Our strength in healthcare innovation empowers us to build a world where complex diseases are prevented, treated, and cured, where treatments are smarter and less invasive, and solutions are personal.Through our expertise in Innovative Medicine and MedTech, we are uniquely positioned to innovate across the full spectrum of healthcare solutions today to deliver the breakthroughs of tomorrow, and profoundly impact health for humanity.Our group specifically generates cell lines that produce large molecule therapeutics in support of the fast-growing pipeline for these various therapeutic areas.As a member of the cell line development team, the candidate will play a crucial role in advancing technology for the next generation of biologics production cell lines.They will plan and execute experiments to overcome limitations of current protein expression systems, focusing on cellular pathways to enhance the quantity and quality of therapeutic proteins.The responsibilities would include:Developing mammalian cell lines secreting complex proteins, including transfection, clone characterization, and bioreactor evaluation.Cell culture development for all protein therapeutics.Evaluating, developing, and implementing new technologies in cell biology, molecular biology, or equipment to improve protein yields and shorten timelines for recombinant protein-secreting CHO cells.Genetic engineering of CHO cells including gene knockouts or knock-ins using CRISPR or YACs/BACs.Familiarity with analyzing genomic, transcriptomic, and proteomic data outputs to identify targets for gene regulation.Experience in optimizing biosynthetic pathways utilizing cutting-edge genomics, transcriptomics, protein engineering, and gene editing tools.Participating in projects from Lead Optimization to New Molecular Entity declaration within a cross-functional environment.Designing and performing experiments for the development of non-natural amino acid (nnAA) expression technology.Qualifications Required:Ph.D. in Cell Biology, Molecular Biology, or related scientific field with 0-3 years' experience in cell culture, protein expression; OR a minimum of 4-6 years of experience beyond a B.Sc./M.Sc. degree.Proven track record of versatility in cell engineering & cell line development.Thorough understanding of RNA biology and network is required.Experience developing mammalian cell lines secreting complex proteins, including transfection, clone characterization, and bioreactor evaluation is required.Ability to work in a matrix environment and connect with different functional groups and people at multiple levels.Excellent verbal and written communication skills
