DescripciónLoxo@Lilly Oncology and CNIO are collaborating on the discovery of new targets for the development of potential cancer therapeutics. We are seeking to recruit a Laboratory Research Associate. The candidate will integrate in a group of 10 scientists at CNIO to evaluate and validate new cancer targets in the emerging areas of Chromosomal Inestability, DNA Repair, Tumor Metabolism, Immunotherapy, Cell signaling and Translational research.ResponsibilitiesSelected candidates will work in collaboration with several investigators for the design and execution of experiments towards target validation in the following research disciplines:Molecular biology: experiments involving molecular biology particularly CRISPR technology including CRISPR library screening, cloning in lentiviral and retroviral expression systems, gene silencing using shRNAs and the CRISPR system, and protein analysis mostly Western blot and IP techniques.Cellular biology: Tumor cell culture and primary culture including the preparation of mouse embryonic fibroblast, luciferase reporter assays, confocal immunofluorescence, high-throughput screening.Bioinformatics and biostatistics: Knowledge in the use of public databases, data mining and analysis.In vivo work: candidates will need to demonstrate experience in animal husbandry, mouse models, with special emphasis in GEM models, xenografts/allografts models both classical and orthotopic as well as in vivo imaging.Immunohistochemistry: sample handling and analysis and imaging analysis.Bioenergetics: Seahorse analysis, metabolic flows by LC-MS and RMN.Criterios de evaluación– At least 4 years of practical experience in genetic and molecular biology: gene silencing, library screening, PCR, isolation of nucleic acids and cloning, Western blot, immunoprecipitation and histology tools to analyze tissues from xenograft mouse models.– Experience in cell culture techniques: Cell culture of tumor cells lines as well as primary culture of mouse embryonic fibroblast, human PBMC and mouse immune cells, ELISA, Immunofluorescence, Flow cytometry techniques and immunophenotyping.– Bioinformatics: handling of public databases, data mining and analysis. Biostatistics.– Husbandry of mouse colonies and animal handling particularly administration of compounds using different protocols and sampling of biological fluids, animal necropsy, in vivo imaging.– Immunohistochemistry and image analysis.– Bioenergetics: Seahorse analysis, metabolic flows by LC-MS and RMN.– Fluent in English.Se ofrece– An opportunity to be part of one of the few European Cancer Research Centers of excellence that effectively combines basic and applied research.– An energetic working environment with a multidisciplinary approach to drug discovery, equipped with state-of-the-art technology.– A competitive salary commensurate with the candidate's experience and qualifications as well as an attractive benefits package.– Contract linked to a project.#J-18808-Ljbffr
