Organisation/Company Principe Felipe Research Center Research Field Biological sciences » Biology Chemistry » Biochemistry Pharmacological sciences » Pharmacy Researcher Profile First Stage Researcher (R1) Positions PHD Positions Country Spain Application Deadline 25 Jul 2024 - 12:00 (Europe/Madrid) Type of Contract Temporary Job Status Full-time Hours Per Week 37.5 Offer Starting Date 1 Jan 2025 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No
Offer Description By resolution of June 28, 2024 of the General Director of Science and Research of the Department of Education, Universities and Employment of the Generalitat Valenciana, the CIPF Neurobiology Laboratory has obtained a pre-doctoral contract from the Santiago Grisolía program (GRISOLIA/2024 ).
The contract is for pre-doctoral research personnel who have obtained a university degree bachelor's degree, architecture, engineering, degree, technical architecture, technical engineering or diploma, or the equivalent in their country, issued by a university or institution not belonging to the Union. European.
The person hired will carry out the doctoral thesis within the framework of the project: Molecular mechanisms of neurological alterations (cognitive and motor) in hyperammonemia and hepatic encephalopathy. Therapeutic implications. In particular in the following objectives:
Identify the mechanisms by which GABAergic neurotransmission modulates peripheral inflammation and neuroinflammation in the cerebellum and hippocampus and its role in cognitive and motor impairment in hyperammonemia.
Identify new pathways and processes involved in cognitive and motor impairment in rats by integrated multi-omics analysis of changes in transcriptomics, proteomics and metabolomics in the brain.
Identify gender differences in cognitive and motor function and induction of hyperammonemia in control rats. Underlying mechanisms.
Identify gender differences in the effects of hyperammonemia on cognitive and motor function and the underlying mechanisms.
Some of the tasks to be carried out within the framework of these studies are:
Flow cytometry to characterize the immunophenotype
Isolation and culture of PBMCs and CD4+ T lymphocytes from rat blood
Carrying out ex vivo experiments in sections of the cerebellum and hippocampus: dissection of the brain areas to be studied, making sections using a vibratome or chopper and incubation in a perfusion system with the different treatments to be evaluated
Processing of the tissue (cerebellum and hippocampus) for subsequent analysis (fixation, homogenization)
Analyze activation of microglia and astrocytes by immunohistochemistry
Quantification of the content of inflammatory markers in specific regions of the cerebellum and hippocampus using immunohistochemistry and immunofluorescence
RNA extraction from tissue and cell samples
Analysis of protein content by Western blot and ELISA assays
Evaluate the membrane expression of neurotransmitter receptors and receptors involved in inflammation
Evaluation of cognitive function in rats using different behavioral tests: motor coordination test (Beam Walking, Catwalk, Motorater), memory and learning test (Y maze, radial maze, location memory test and novel object recognition ) and other complementary tests to analyze fatigue, itching and thermal sensitivity
Use of programs for statistical analysis and data representation: GraphPad Prism, Excel and SPSS.
Preparation of results for presentation in communications at conferences and in scientific articles.
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