Descripción: The IdiPAZ-CNIO Pediatric Onco-Hematology Clinical Unit, within the Clinical Research Programme, is seeking to recruit a Predoctoral Research Assistant.Roles and responsibilities of the position:Design and development of experiments.Analyzing data and writing manuscripts.Communicate data to other laboratories to make good collaborations.Participate in formative activities related to the field of pediatric cancer and immunotherapy.Disseminate knowledge in international and national conferences.Criterios de evaluación– Specific qualifications:Degree: BSc in Biochemistry.Master: MSc in Biomedicine.One year of experience as a lab technician in National Hospital of Paraplejics.PhD in Biomedicine: 3 years of experience (ongoing).Animal experimentation certificate (functions A, B, C and D).7 years at least working in a research laboratory.One year working in the neuroscience/neurobiology field.Knowledge in cellular immunotherapy.Worked and published in the field of pediatric cancer.3 years at least working in the CAR-T cells field.3 years working on extracellular vesicles (exosomes and microvesicles).At least 3 months of internship in CAR-T cells and blood-brain barrier cells in foreign laboratories.At least one first author publication in an international journal, in the field of CAR-T cells.Oral publications on international conferences.English is compulsory.
The candidate must be fluent in English with excellent communication and writing skills.Course of Statistics and R programming.Microscopy and Image Analysis Course.Introduction Course in Flow Cytometry.Course on extracellular vesicles.Course on advanced therapies for clinical uses.Technical skills:Handling of experimental animals: mice.
Genetically engineered mice genotyping, organ extraction and processing.
Subcutaneous, intravenous, intracranial, retroorbital and intraperitoneal injections, euthanasia.
Patient and cell lines Derived Xenografts for drug and cell therapy testing.In vitro assays: Culture of primary human and mice cells.
Cell lines culture.
Depletion and selection of T cells, T cell subpopulations.
Sphere Formation assays from cell lines.Immunoassays: Enzymatic and Luminiscent Techniques.
ELISA.
Bioplex.Europium-TDA: T cell cytotoxicity assays.Activation and expansion of human T cells.Lentivirus production and handling.Transduction of primary cells (T cells) and cell lines.Multicolor Flow cytometry: Analysis of superficial and intracellular markers.
Cell cycle and cell division studies.Isolation and characterization of exosomes derived from cell lines and human T cells.Molecular biology: Western Blot and protein analysis from cell lines, human primary cells (T cells) and exosomes, qPCR and quantitative PCR.Fluorescence and confocal microscopy, analysis of human blood-brain barrier cells and human T cells, different cell lines.Evaluation of immunological synapses between human T cells and tumor cells.Immunohistochemistry/immunofluorescence.Handling with ultracentrifuges, vibratome, cryotome (for brain slices).Ex vivo assays: organotypic cultures of brain slices from human glioblastoma mouse model to test the efficacy of human T cells and exosomes.Good knowledge of MS Word, Excel, experience in literature searching and capable of giving clear scientific presentations using PowerPoint.Programs: Minitab, Image J, GraphPad Prism, Image Lab, Mendeley, Cytoscape, MATLAB.Se ofrece:The opportunity to be part of one of the few European Cancer Research Centres of excellence.An excellent working, multidisciplinary environment.Competitive salary.Contract linked to a project.El anuncio original lo puedes encontrar en Kit Empleo:https://www.kitempleo.es/empleo/129652683/predoctoral-research-assistant-for-the-idipaz-cnio-pediatric-onco-hematology-clinical-unit-aiprepoh-yf548-madrid/?utm_source=html #J-18808-Ljbffr