.Created in 2005 by the Generalitat de Catalunya (Government of Catalonia) and the University of Barcelona, IRB Barcelona is a Severo Ochoa Centre of Excellence—a seal that was awarded in 2011. The institute is devoted to conducting research of excellence in biomedicine and to transferring results to clinical practice, thus improving people's quality of life, while simultaneously promoting the training of outstanding researchers, technology transfer, and public communication of science. Its 29 laboratories and seven core facilities address basic questions in biology and are orientated to diseases such as cancer, metastasis, Alzheimer's, diabetes, and rare conditions.IRB Barcelona is an international centre that hosts 500 members and 45 nationalities. It is located in the Barcelona Science Park. IRB Barcelona forms part of the Barcelona Institute of Science and Technology (BIST) and the "Xarxa de Centres de Recerca de Catalunya" (CERCA). This international consortium has developed cutting-edge microscopy tools that contribute to the understanding of how cancer cells behave in complex 3D structures and enable robust interrogation of responses to cancer therapy with single cell resolution. Screens will include longitudinal live cell imaging assays and use sophisticated phenotypic endpoints - with the potential to link to and inform subsequent single cell genomic and proteomic analysis. This project is financed by CRUK-AECC (GEACC19006BAT; from the Accelerator Awards of the "Fundación de la Asociación Española contra el Cancer (FAECC)").KEY PUBLICATIONS RELATED TO THE PROJECT Cañellas-Socias, et al and Batlle E. Metastatic recurrence in colorectal cancer arises from residual EMP1+ cells. Nature. Nov; 611(7936).Alvarez-Varela, et al. and Batlle E. Mex3a marks drug-tolerant persister colorectal cancer cells that mediate relapse after chemotherapy. Nature Cancer. Sep; 3(9):1052-1070. 2022.Herpes et al. and Batlle E, Throsby M. Functional screening on patient-derived organoids identifies a therapeutic bispecific antibody that triggers EGFR degradation in LGR5+ tumor cells. Nature Cancer .Morral et al. and Batlle E. Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell. 26: 845-861. 2020.Cortina C, et al. and Batlle E. A genome editing approach to study cancer stem cells in human tumors. EMBO Molecular Medicine. 9 (7): 869-879. 2017.Tosi S, Bardia L, Filgueira MJ, Calon A and Colombelli J. LOBSTER: An environment to design bioimage analysis workflows for large and complex fluorescence microscopy data. Bioinformatics. 36(8):2634-2635. 2020.DUTIES Conduct independent research in the area of advanced microscopy applied to research with organoids, including novel spinning-disk and lightsheet-based high-throughput instruments.Standardize advanced microscopy workflows for organoids screening across four European facilities
