CRAG is an independent research institution engaged in leading-edge basic and applied plant and farm animal sciences. CRAG is established as a Consortium of the Spanish National Research Council (CSIC), Institute of Agrifood Research and Technology (IRTA), Autonomous University of Barcelona (UAB), and University of Barcelona (UB). The Center is located at the UAB Campus and currently hosts 200 members from across the world.
The Center for Research in Agricultural Genomics (CRAG) is looking for a PhD Student to be part of the group Protein regulation in development and stress.
The candidate will be participating in:
Cloning of fluorescent protein fusions with SUMOylation machineryE.coli transformation and protein purificationProtein analysis techniques for the generated proteins, such as SDS-page, western blot, native gelsFluorescence Resonance Energy Transfer (FRET)Plant production (A. thaliana) and protein purification from tissuesPlant RNA extraction, RNA-seq preparation, and data analysiscDNA synthesis and quantitative PCR analysisThe candidate is expected to conduct research under the supervision of Dr. L. Maria Lois including:
Day to day responsibility for the conduct and satisfactory progress of the research;Keep meticulous experimental records, analyze data, interpret reports and bring new insights to research area;Keep up to date with the relevant literature, evaluate and criticize texts;Contribute ideas and be involved with the longer-term planning of the project;Work safely and responsibly with regard to CRAG rules;Participate in laboratory organization and function;Contribute to group meetings;The tasks are included within the framework of the project PID2021-123572NB-I00 "Study of signaling networks linked to cytosolic SUMOylation facilitated by SUMO activating enzyme processing in plants (SUMOcell)", funding by MICIU/AEI/ 10.13039/501100011033, "ERDF A way of making Europe".
REQUIREMENTS: Educational requirements: Degree/master in Biochemistry or similar.
Skills:
Critical thinking and creativity, organization and planning, resilienceStrong analytical capacityAbility to build relationships and collaborate with others, internally and externallyWriting and oral communicationWillingness to learn new experimental/intellectual techniquesMotivation and commitmentExperience: Previous lab work applying biochemical, molecular biology, and microscopy techniques.
CRAG is committed to the principles of the Code of Conduct for the Recruitment of Researchers of the European Commission. All candidates will be considered qualified for the job without restrictions of gender, race, nationality or disability.
Integration policy: reserve the place in a first turn for disabled staff.
Interested candidates must submit the following documents:
A motivation letter, including a brief summary of work experience and a statement of research interests and career goals (2 pages maximum).Student's certified Academic Record, including a detailed record of study / transcript (a list of attended courses and corresponding grades).Any additional files considered relevant to the application, but please only provide documents that are important to support it two reference letters from university lecturers or scientists with whom the applicant has studied or worked.
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