.You are here:Home > Lab Technician – Deep Mutational ScanningLab Technician – Deep Mutational ScanningThe InstituteThe Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG is composed of an interdisciplinary, motivated and creative scientific team supported by a flexible and efficient administration and high-end innovative technologies.We are looking to hire a Lab Technician with experience in deep mutational scanning and data analysis to perform large-scale selection experiments to better understand and predict stop codon readthrough and nonsense mediated mRNA decay.About the team/lab/departmentThe focus of Lehner lab is using massively parallel selection experiments to tackle the fundamental encoding problems of molecular biology. Towards this goal, we have developed, benchmarked, and applied at scale experimental methods that use DNA synthesis-selection-sequencing to perform biophysical measurements including quantifying how changes in sequence alter protein stability, binding affinity, aggregation, allostery, and expression. We have used these methods to produce the first comprehensive maps of long-range allosteric communication in proteins, to quantify the effects of over 500,000 variants in over 500 human proteins, and to probe the genetic architecture of proteins, protein interactions, and RNAs. A key component of this work has been the development of computational methods for analyzing deep mutational scanning data and for fast and flexible fitting of mechanistic and statistical models to the data. Our current focus is on generating datasets of sufficient size and diversity to train machine learning models to accurately predict how changes in sequence alter molecular biology.Whom would we like to hire?Professional experienceMust HaveYou have over 4 years of experience in molecular biology and experimental assay development.You have proven experience in deep mutational scanning or related approaches.Experience in statistical data analysis and bioinformatics.Desirable but not required/Nice to haveExperience in high-throughput genomics.Education and trainingLanguagesYou are proficient in English.Technical skillsYou have advanced molecular biology skills and the ability to analyze large sequence datasets.CompetencesYou can work in an interdisciplinary team.You can plan, optimize, and execute complex protocols.You can work independently and have experience in project management.You have very good organizational skills.The Offer – Working ConditionsEstimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales.We provide a highly stimulating environment with state-of-the-art infrastructures and unique professional career development opportunities. To check out our training and development portfolio, please visit our website in the training section
