.As SpliceBio is growing, we're looking for exceptional professionals to be part of our team, supporting the development of gene therapies for diseases for which currently no treatment exists. We are now hiring a Scientist Vector engineering for AAV Gene Therapy. In this capacity you will be responsible for leading an ambitious scientific vision and strategy for internal AAV gene therapy vector genome research. Moreover the successful candidate will be empowered to develop and implement the strategic view for the next generation of gene therapy platforms and programs, working in close collaboration with the Head of Translational and Chief Development Officer.The successful candidate will have a strong background and hands-on experience working in molecular biology, AAV vector engineering, genetic engineering techniques e.G. CRISPR, PCR, cloning, FACS, transfection, transduction, and assay development and also have strong knowledge in the regulatory DNA elements that can be modified to improve transgene expression. Production and purification of AAVs is a plus.He/She will work collaboratively with cross-functional teams and external third partners, ensuring the highest standards of data quality and regulatory compliance are met throughout the development process.Reporting to our Head of Translational, this is what you'll be working on:
- Lead AAV construct design-related activities, drive innovation in AAV cargo design.
- Ensure the successful design of the AAV skeleton to improve expression of the transgene in the desired target cells.
- Hands-on work in the cloning and screening of the AAV vectors (e.G. cell culture transfection and transduction and characterization of the constructs).
- Perform and/or oversee sample analysis and data interpretation.
- Works closely with external partners on AAV viral vector optimization, ensuring the timely completion of projects and the delivery of high-quality data.
- Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods.
- Actively participates as a member of multifunctional project teams. Collaborate with cross- functional teams, including research and development, as well as external third parties, to ensure seamless integration of data into product development.RequirementsFor a successful fulfilment of this position, we are interested in candidates meeting the following requirements:
- PhD in virology, virus bioengineering, gene/cell therapy, molecular biology or related field.
- At least 4 years of practical experience in the industry/academic field, with a focus on AAV gene therapy.
- Experience with AAV plasmid design and cloning and Mammalian cell/tissue culture (including transfection and/or transduction).
- Experience with a variety of cellular and molecular biology techniques such as qPCR/ddPCR, flow cytometry, Western Blot, etc
