Offer DescriptionChromatographic techniques for protein purification: gel-filtration, ion-exchange, hydrophobic, IMAC, and reverse-phase.In vitro handling of proteins and other biomolecules.Expression of proteins in bacterial systems.Site-directed mutagenesis (Basic PCR techniques).Basic microbiology techniques (preparation and handling of media, cell growth, antibiotic preparation).Western blots.Cell growth assays (MTT), immunofluorescence assays, and proximity assays (PLA) in cell media (studies in cellulose).Handling of FPLC and HPLC for biomolecule purification (or their complexes) and determination of their molecular sizes.DNA and protein electrophoresis (in native and denatured states) in gels with different compositions.Use of different electroscopic techniques: fluorescence, visible ultraviolet, circular dichroism, nuclear magnetic resonance.Interpretation of spectra from these techniques.Use of different biophysical techniques: dynamic light scattering, bio-layer interferometry, differential scanning calorimetry, and isothermal titration calorimetry.Interpretation of results.Determination of kinetic parameters (of enzymatic reactions, binding reactions, folding reactions, and/or chemical reactions in general) with electroscopic and biophysical techniques.Interpretation of these parameters.Analysis, interpretation, and representation of data with programs such as Microsoft Office (Excel, Word, PowerPoint), SigmaPlot, Origin, and/or KaleidaGraph.Where to apply: E-mail ******Field: OtherEducation Level:Bachelor Degree or equivalentMaster Degree or equivalentSkills/QualificationsA Research experience related to the project (80 points)Official academic qualifications (bachelor, master, doctorate) - Bachelor or equivalent of: biotechnology, biology, chemistry, pharmacy, biomedicine, biotechnological engineering, biochemical engineering, food technology.
(Accounting for 30 points)Master: of value are masters related to biotechnology, biology, chemistry, pharmacy, biomedicine, biotechnological engineering, biochemical engineering, food technology, and those similar.
(Accounting for 10 points)Knowledge of languages (5 points)Research experience (participation in other projects) (10 points)Quality, number of publications, and degree of participation/results of protected research (5 points)Experience and training in managing techniques and methods necessary for the execution of the project (10 points)Stays at universities and/or other research centers (5 points)Other merits to assess (5 points)Teaching experience (10 points)Previous professional activities related to the area of research the project lies within (10 points)MAXIMUM SCORE: 100MINIMUM SCORE TO BE ELIGIBLE: 60In the case of a tie between two candidates, a personal interview will be given.#J-18808-Ljbffr